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The positive sense single stranded RNA genome is packaged in the capsid which is formed by the capsid protein.
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Once the blocking solution has adequate contact time with the blot, a specific competitor RNA is applied and given time to incubate at room temperature.
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The three members of this genus are positive-stranded RNA viruses with a multipartite genome (3-4 parts).
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Product proteins from the processing includes various replicative enzymes such as RNA dependent polymerase, RNA helicase, and proteinase.
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Coronaviruses contain the largest known viral RNA genome in number of nucleotides, of approximately 30,000 bases.
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Stem-loop or hairpin loop is the most common element of RNA secondary structure.
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Its genome is about 29.7kb, which is one of the largest among RNA viruses.
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Analyses of the genome sequences resulted in the discovery of a conserved RNA motif.
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Synthetic RNA thermometers have been designed with just a simple single-hairpin structure.
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This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis.
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